Review



biotinylated antiavidin d antibody  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Vector Laboratories biotinylated antiavidin d antibody
    SatIII RNAs mark a long period of recovery from stress. ( A ) Total RNAs were extracted from unstressed HeLa cells (C) and from cells heat shocked for 1 h at 42 °C and then allowed to recover at 37 °C for the indicated time periods. The expression level of SatIII RNAs was determined using real time PCR, as described , and expressed as a function of the basal level observed in control cells. The histogram represents the average of three independent experiments ± standard deviation. ( B ) The same cell populations were analyzed using in situ RNA FISH with a <t>biotinylated</t> oligo against SatIII RNAs, as previously described . Cell nuclei were stained using DAPI. ( C ) Short RNAs were prepared from HeLa cells treated as in panel A, separated on 5% polyacrylamide gels and analyzed using Northern blotting with a locked nucleic acid (LNA) oligo specific for SatIII repeats. M: molecular size markers. The same filter was hybridized to a probe specific for Mir16 as a loading control.Bars: 10 µm.
    Biotinylated Antiavidin D Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 9821 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+antiavidin+d+antibody/Biotinylated+Anti-Avidin+Antibody/pmc07216065-218-15-19
    Average 96 stars, based on 9821 article reviews
    biotinylated antiavidin d antibody - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Heat Shock Affects Mitotic Segregation of Human Chromosomes Bound to Stress-Induced Satellite III RNAs"

    Article Title: Heat Shock Affects Mitotic Segregation of Human Chromosomes Bound to Stress-Induced Satellite III RNAs

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21082812

    SatIII RNAs mark a long period of recovery from stress. ( A ) Total RNAs were extracted from unstressed HeLa cells (C) and from cells heat shocked for 1 h at 42 °C and then allowed to recover at 37 °C for the indicated time periods. The expression level of SatIII RNAs was determined using real time PCR, as described , and expressed as a function of the basal level observed in control cells. The histogram represents the average of three independent experiments ± standard deviation. ( B ) The same cell populations were analyzed using in situ RNA FISH with a biotinylated oligo against SatIII RNAs, as previously described . Cell nuclei were stained using DAPI. ( C ) Short RNAs were prepared from HeLa cells treated as in panel A, separated on 5% polyacrylamide gels and analyzed using Northern blotting with a locked nucleic acid (LNA) oligo specific for SatIII repeats. M: molecular size markers. The same filter was hybridized to a probe specific for Mir16 as a loading control.Bars: 10 µm.
    Figure Legend Snippet: SatIII RNAs mark a long period of recovery from stress. ( A ) Total RNAs were extracted from unstressed HeLa cells (C) and from cells heat shocked for 1 h at 42 °C and then allowed to recover at 37 °C for the indicated time periods. The expression level of SatIII RNAs was determined using real time PCR, as described , and expressed as a function of the basal level observed in control cells. The histogram represents the average of three independent experiments ± standard deviation. ( B ) The same cell populations were analyzed using in situ RNA FISH with a biotinylated oligo against SatIII RNAs, as previously described . Cell nuclei were stained using DAPI. ( C ) Short RNAs were prepared from HeLa cells treated as in panel A, separated on 5% polyacrylamide gels and analyzed using Northern blotting with a locked nucleic acid (LNA) oligo specific for SatIII repeats. M: molecular size markers. The same filter was hybridized to a probe specific for Mir16 as a loading control.Bars: 10 µm.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Standard Deviation, In Situ, Staining, Northern Blot

    Related Articles

    Amplification:

    Article Title: Heat Shock Affects Mitotic Segregation of Human Chromosomes Bound to Stress-Induced Satellite III RNAs
    Article Snippet: .. The biotinylated probe was detected with FITC-avidin (Vector Laboratories) and the signal was amplified with biotinylated antiavidin D antibody (Vector Laboratories) followed by FITC-avidin. .. Nuclei were stained with 0.1 μg/mL 4′-6-diamidino-2 phenylindole (DAPI, Sigma-Aldrich).

    Fluorescence:

    Article Title: Chemokine receptor expression and chemotactic responsiveness of human monocytes after influenza A virus infection.
    Article Snippet: Chemokine receptor surface expression Cells were incubated with biotinylated anti-human CCR1, CCR2, CCR5 (R&D Systems, Wiebaden, Germany), or isotype-specific goat anti-mouse (PharMingen, Hamburg, Germany) monoclonal antibody, washed twice, and subsequently incubated with streptavidin-phycoerythrin (PE; PharMingen). .. To amplify the received fluorescence signal, cells were further incubated with biotinylated antiavidin D antibody (Vector Laboratories, Burlingame, CA), followed by a second incubation with streptavidin-PE conjugate. .. The samples were analyzed on a FACScan flow cytometer (Becton Dickinson, Heidelberg, Germany).

    Incubation:

    Article Title: Chemokine receptor expression and chemotactic responsiveness of human monocytes after influenza A virus infection.
    Article Snippet: Chemokine receptor surface expression Cells were incubated with biotinylated anti-human CCR1, CCR2, CCR5 (R&D Systems, Wiebaden, Germany), or isotype-specific goat anti-mouse (PharMingen, Hamburg, Germany) monoclonal antibody, washed twice, and subsequently incubated with streptavidin-phycoerythrin (PE; PharMingen). .. To amplify the received fluorescence signal, cells were further incubated with biotinylated antiavidin D antibody (Vector Laboratories, Burlingame, CA), followed by a second incubation with streptavidin-PE conjugate. .. The samples were analyzed on a FACScan flow cytometer (Becton Dickinson, Heidelberg, Germany).



    Similar Products

    biotinylated antiavidin d antibody  (Vector Laboratories)
    96
    Vector Laboratories biotinylated antiavidin d antibody
    SatIII RNAs mark a long period of recovery from stress. ( A ) Total RNAs were extracted from unstressed HeLa cells (C) and from cells heat shocked for 1 h at 42 °C and then allowed to recover at 37 °C for the indicated time periods. The expression level of SatIII RNAs was determined using real time PCR, as described , and expressed as a function of the basal level observed in control cells. The histogram represents the average of three independent experiments ± standard deviation. ( B ) The same cell populations were analyzed using in situ RNA FISH with a <t>biotinylated</t> oligo against SatIII RNAs, as previously described . Cell nuclei were stained using DAPI. ( C ) Short RNAs were prepared from HeLa cells treated as in panel A, separated on 5% polyacrylamide gels and analyzed using Northern blotting with a locked nucleic acid (LNA) oligo specific for SatIII repeats. M: molecular size markers. The same filter was hybridized to a probe specific for Mir16 as a loading control.Bars: 10 µm.
    Biotinylated Antiavidin D Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+antiavidin+d+antibody/Biotinylated+Anti-Avidin+Antibody/pmc07216065-218-15-19
    Average 96 stars, based on 1 article reviews
    biotinylated antiavidin d antibody - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    biotin conjugated antiavidin d antibody  (Vector Laboratories)
    96
    Vector Laboratories biotin conjugated antiavidin d antibody
    SatIII RNAs mark a long period of recovery from stress. ( A ) Total RNAs were extracted from unstressed HeLa cells (C) and from cells heat shocked for 1 h at 42 °C and then allowed to recover at 37 °C for the indicated time periods. The expression level of SatIII RNAs was determined using real time PCR, as described , and expressed as a function of the basal level observed in control cells. The histogram represents the average of three independent experiments ± standard deviation. ( B ) The same cell populations were analyzed using in situ RNA FISH with a <t>biotinylated</t> oligo against SatIII RNAs, as previously described . Cell nuclei were stained using DAPI. ( C ) Short RNAs were prepared from HeLa cells treated as in panel A, separated on 5% polyacrylamide gels and analyzed using Northern blotting with a locked nucleic acid (LNA) oligo specific for SatIII repeats. M: molecular size markers. The same filter was hybridized to a probe specific for Mir16 as a loading control.Bars: 10 µm.
    Biotin Conjugated Antiavidin D Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+antiavidin+d+antibody/Biotinylated+Anti-Avidin+Antibody/pmc03510517-75-30-34
    Average 96 stars, based on 1 article reviews
    biotin conjugated antiavidin d antibody - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    biotinylated goat antiavidin d antibody  (Vector Laboratories)
    96
    Vector Laboratories biotinylated goat antiavidin d antibody
    SatIII RNAs mark a long period of recovery from stress. ( A ) Total RNAs were extracted from unstressed HeLa cells (C) and from cells heat shocked for 1 h at 42 °C and then allowed to recover at 37 °C for the indicated time periods. The expression level of SatIII RNAs was determined using real time PCR, as described , and expressed as a function of the basal level observed in control cells. The histogram represents the average of three independent experiments ± standard deviation. ( B ) The same cell populations were analyzed using in situ RNA FISH with a <t>biotinylated</t> oligo against SatIII RNAs, as previously described . Cell nuclei were stained using DAPI. ( C ) Short RNAs were prepared from HeLa cells treated as in panel A, separated on 5% polyacrylamide gels and analyzed using Northern blotting with a locked nucleic acid (LNA) oligo specific for SatIII repeats. M: molecular size markers. The same filter was hybridized to a probe specific for Mir16 as a loading control.Bars: 10 µm.
    Biotinylated Goat Antiavidin D Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+antiavidin+d+antibody/Biotinylated+Anti-Avidin+Antibody/10__2203_slash_dose___response__11___002__rithidech-91-11-16
    Average 96 stars, based on 1 article reviews
    biotinylated goat antiavidin d antibody - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    biotinylated goat antiavidin d  (Vector Laboratories)
    96
    Vector Laboratories biotinylated goat antiavidin d
    SatIII RNAs mark a long period of recovery from stress. ( A ) Total RNAs were extracted from unstressed HeLa cells (C) and from cells heat shocked for 1 h at 42 °C and then allowed to recover at 37 °C for the indicated time periods. The expression level of SatIII RNAs was determined using real time PCR, as described , and expressed as a function of the basal level observed in control cells. The histogram represents the average of three independent experiments ± standard deviation. ( B ) The same cell populations were analyzed using in situ RNA FISH with a <t>biotinylated</t> oligo against SatIII RNAs, as previously described . Cell nuclei were stained using DAPI. ( C ) Short RNAs were prepared from HeLa cells treated as in panel A, separated on 5% polyacrylamide gels and analyzed using Northern blotting with a locked nucleic acid (LNA) oligo specific for SatIII repeats. M: molecular size markers. The same filter was hybridized to a probe specific for Mir16 as a loading control.Bars: 10 µm.
    Biotinylated Goat Antiavidin D, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+antiavidin+d+antibody/Biotinylated+Anti-Avidin+Antibody/10__1086_slash_344556-78-11-19
    Average 96 stars, based on 1 article reviews
    biotinylated goat antiavidin d - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    biotinylated antiavidin d antibodies  (Vector Laboratories)
    96
    Vector Laboratories biotinylated antiavidin d antibodies
    SatIII RNAs mark a long period of recovery from stress. ( A ) Total RNAs were extracted from unstressed HeLa cells (C) and from cells heat shocked for 1 h at 42 °C and then allowed to recover at 37 °C for the indicated time periods. The expression level of SatIII RNAs was determined using real time PCR, as described , and expressed as a function of the basal level observed in control cells. The histogram represents the average of three independent experiments ± standard deviation. ( B ) The same cell populations were analyzed using in situ RNA FISH with a <t>biotinylated</t> oligo against SatIII RNAs, as previously described . Cell nuclei were stained using DAPI. ( C ) Short RNAs were prepared from HeLa cells treated as in panel A, separated on 5% polyacrylamide gels and analyzed using Northern blotting with a locked nucleic acid (LNA) oligo specific for SatIII repeats. M: molecular size markers. The same filter was hybridized to a probe specific for Mir16 as a loading control.Bars: 10 µm.
    Biotinylated Antiavidin D Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+antiavidin+d+antibody/Biotinylated+Anti-Avidin+Antibody/pm10965124-30-16-20
    Average 96 stars, based on 1 article reviews
    biotinylated antiavidin d antibodies - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    SatIII RNAs mark a long period of recovery from stress. ( A ) Total RNAs were extracted from unstressed HeLa cells (C) and from cells heat shocked for 1 h at 42 °C and then allowed to recover at 37 °C for the indicated time periods. The expression level of SatIII RNAs was determined using real time PCR, as described , and expressed as a function of the basal level observed in control cells. The histogram represents the average of three independent experiments ± standard deviation. ( B ) The same cell populations were analyzed using in situ RNA FISH with a biotinylated oligo against SatIII RNAs, as previously described . Cell nuclei were stained using DAPI. ( C ) Short RNAs were prepared from HeLa cells treated as in panel A, separated on 5% polyacrylamide gels and analyzed using Northern blotting with a locked nucleic acid (LNA) oligo specific for SatIII repeats. M: molecular size markers. The same filter was hybridized to a probe specific for Mir16 as a loading control.Bars: 10 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Heat Shock Affects Mitotic Segregation of Human Chromosomes Bound to Stress-Induced Satellite III RNAs

    doi: 10.3390/ijms21082812

    Figure Lengend Snippet: SatIII RNAs mark a long period of recovery from stress. ( A ) Total RNAs were extracted from unstressed HeLa cells (C) and from cells heat shocked for 1 h at 42 °C and then allowed to recover at 37 °C for the indicated time periods. The expression level of SatIII RNAs was determined using real time PCR, as described , and expressed as a function of the basal level observed in control cells. The histogram represents the average of three independent experiments ± standard deviation. ( B ) The same cell populations were analyzed using in situ RNA FISH with a biotinylated oligo against SatIII RNAs, as previously described . Cell nuclei were stained using DAPI. ( C ) Short RNAs were prepared from HeLa cells treated as in panel A, separated on 5% polyacrylamide gels and analyzed using Northern blotting with a locked nucleic acid (LNA) oligo specific for SatIII repeats. M: molecular size markers. The same filter was hybridized to a probe specific for Mir16 as a loading control.Bars: 10 µm.

    Article Snippet: The biotinylated probe was detected with FITC-avidin (Vector Laboratories) and the signal was amplified with biotinylated antiavidin D antibody (Vector Laboratories) followed by FITC-avidin.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Standard Deviation, In Situ, Staining, Northern Blot